Table 1.

List of simulations performed. For details, see Materials and Methods.

Chemical System	Force Field	MD Production Run Length
hAgo2 apo–enzyme	Amber03 ILDN	100 ns
hAgo2_5’-U–guide	AMBER Parmbsc0	100 ns
hAgo2_5’-A–guide	AMBER Parmbsc0	100 ns
hAgo2_5’-C–guide	AMBER Parmbsc0	100 ns
hAgo2_5’-G–guide	AMBER Parmbsc0	100 ns