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. 2015 Dec 29;17(1):35. doi: 10.3390/ijms17010035

Figure 3.

Figure 3

Effect of SD on the expression levels of osteoblastic markers and hST3Gal V. Total RNA from MG-63 cells was isolated after incubation in serum-free medium for the indicated time periods (A) or after culture for 24 h in medium containing various concentration of FBS (B), and mRNA transcripts of osteoblastic markers and hST3Gal V were detected by reverse transcription-polymerase chain reaction (RT-PCR). As an internal control, parallel reactions were performed to measure the levels of the housekeeping gene β-actin; (C) Equal amounts of cell lysates (20 μg) were separated on sodium dodecyl sulfate (SDS)-polyacrylamide gels and transferred to a polyvinylidene fluoride (PVDF) membrane. The membrane was probed with specific antibodies against Runx2 and hST3Gal V. GAPDH was used as an internal control.