Figure 5.

Effect of Ca²⁺ chelator (EGTA) and CaM antagonists (CPZ and TFP) pre-treatment on CaM levels in NPPB-treated tea roots, respectively. (A) Control, NPPB, EGTA and Ca²⁺ indicate the application of distilled water, 50 µM NPPB, 1 mM EGTA and 1 mM Ca²⁺ for 2 h, respectively; EGTA + NPPB refers to tea roots were placed in 1 mM EGTA for 0.5 h, then directly subjected to 50 µM NPPB for 2 h; (B) Control, NPPB and CPZ, TFP indicate distilled water, 50 µM NPPB and 50 µM CPZ or TFP for 2 h, respectively; CPZ + NPPB and TFP + NPPB refer to tea roots were placed in 50 µM CPZ and TFP for 0.5 h respectively, then subjected to a direct 50 µM NPPB for 2 h. CaM level was detected after control, NPPB and CPZ, TFP, CPZ + NPPB and TFP + NPPB treatment in tea roots. Data indicate mean ± SD (n = 4). Error bars indicate differences among the treatments and different low case numbers near the chart bars indicate the level of significance as compared with control at p < 0.05; Note: The data for Ca²⁺ treatment was adapted from [31].