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Analysis of RNase-L association with the cytoskeleton. Caco-2 cells were treated with 500 U/mL hIFNα for 24 h and then infected with encephalomyocarditis virus (EMCV; MOI = 10) for 2 and 4 h. Soluble and cytoskeletal protein fractions were collected using the ProteoExtract Cytoskeleton Enrichment and Isolation Kit (Millipore) according to the manufacturer’s protocol. GAPDH and vimentin were used to evaluate soluble and cytoskeleton fraction purity, respectively.
