Figure 3.

Transmission of atypical GSS P102L into 101LL mice. Intracerebral inoculation of partially purified PrP^Sc (brain homogenate) from a patient with atypical GSS P102L into 101LL transgenic mice resulted in multicentric PrP-positive plaques in the corpus callosum and periventricular zone of 101LL mice at >600 days post inoculation (dpi). Two subsequent subpassages using infected 101LL mouse brain homogenate also showed multicentric PrP-positive plaques, again at 600 dpi. No clinical signs of prion disease or vacuolar pathology were observed in any mice despite the presence of PrP-amyloid plaques. PrP-amyloid stained with anti PrP Mab 6H4 [169]. Arrows indicate PrP amyloid plaques.