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. 2016 Jan 15;17(1):112. doi: 10.3390/ijms17010112

Figure 4.

Figure 4

Sp1 stimulates the expression of porcine ROCK1 gene. (A) Over-expression of Sp1 up-regulated ROCK1 luciferase activity; (B) Over-expression efficacy of Sp1; (C,D) Over-expression of Sp1 stimulated ROCK1 expression at mRNA and protein level; (E) The interferences efficacy of siRNA; (F) Suppressing Sp1 reduced the ROCK1 promoter activity; (G,H) Inhibition of Sp1 suppressed ROCK1 expression at mRNA and protein level. The amount of plasmid was kept constant by the addition of pcDNA3.1 (+) vector. The data were obtained both in PK and C2C12 cells and expressed as means ± SD of three replicates. * p < 0.05, ** p < 0.01.