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. 2016 Jan 16;17(1):120. doi: 10.3390/ijms17010120

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) mRNA expression in IL-1α-treated meniscal cells with/without PRP. mRNA expression of aggrecan and type II collagen was generally low compared to that observed in IL(+). The aggrecan and type II collagen expression levels were lower in IL(+)PRP(+) than IL(+); (B) Immunoblot analysis in IL-1α-treated culture media of meniscal cells with/without PRP. At every time point, protein expression levels of MMP-1, MMP-3, and TIMP-1 were higher in IL(+)PRP(+) than in IL(+), and there was no significant difference in their time courses. * p < 0.05; ** p < 0.01 versus Day 3. ^# p < 0.05; ^## p < 0.01; ^### p < 0.001 versus IL(+).

(A) mRNA expression in IL-1α-treated meniscal cells with/without PRP. mRNA expression of aggrecan and type II collagen was generally low compared to that observed in IL(+). The aggrecan and type II collagen expression levels were lower in IL(+)PRP(+) than IL(+); (B) Immunoblot analysis in IL-1α-treated culture media of meniscal cells with/without PRP. At every time point, protein expression levels of MMP-1, MMP-3, and TIMP-1 were higher in IL(+)PRP(+) than in IL(+), and there was no significant difference in their time courses. * p < 0.05; ** p < 0.01 versus Day 3. ^# p < 0.05; ^## p < 0.01; ^### p < 0.001 versus IL(+).