Table 1.
The primer sequences provided in the study.
| Category | Primer | Gene Bank | Size (bp) | Sequence (5′-3′) |
|---|---|---|---|---|
| Chondrogenesis | GAPDH | DQ403051.1 | 304 | TCA CCA TCT TCC AGG AGC GA |
| CAC AAT GCC GAA GTG GTC GT | ||||
| Aggrecan | XM002723376.1 | 744 | CTC ACC CCG AGA ATC AAA TG | |
| AGG AGG TTT CCG CCG CAG TT | ||||
| Type I Collagen | XM 002713800.1 | 310 | CGC GAT GGT CAG CCT GGA CA | |
| CCG GGA GGG CCA GCA GGA CC | ||||
| Type II Collagen | D 83228.1 | 370 | GAC CCC ATG CAG TAC ATG | |
| AGC CGC CAT TGA TGG TCT CC | ||||
| Biglycan | XM 002722633.1 | 386 | CAC TGC CAC CTG CGG GTT GT | |
| TCT AGG GGG TTC CCG CCC ATC | ||||
| Catabolic molecule | MMP-1 | NM 001171139.1 | 553 | GCA ACC CAG GTG TGG AGT GCC |
| TGG GCC TAC TGG CTG ACT GGG | ||||
| MMP-3 | NM 001082280.1 | 339 | CTG GAG GTT TGA TGA GAA GA | |
| CAG TTC ATG CTC GAG ATT CC | ||||
| MMP-9 | NM 001082203.1 | 519 | GAC GGC AAG CCC TGC GAG TT | |
| TGT GGT GGT GGC TGG AGG CT | ||||
| MMP-13 | NM 001082037.1 | 328 | GCA GCA GTC TCC AGG CAC GG | |
| TCA GGG ACC CCG CAT CTC GG | ||||
| Anabolic molecule | TIMP-1 | NM 001082232.2 | 326 | GCA ACT CCG ACC TTG TCA TC |
| AGC GTA GGT CTT GGT GAA GC | ||||
| TIMP-2 | XM 002723776.1 | 460 | AAC GGA GTC TGG TGG TGC ATT CC | |
| CTT GGC CTG GTG CCC GTT GAT | ||||
| Growth factor | TGF-β | AB 020217.1 | 231 | GCA AGG ACC TGG GCT GGA A |
| AGT AAC ACG ATG GGC AGT GGC | ||||
| VEGF | XM 002714697.1 | 100 | CGC AGC TAC TGC CAG CCG AT | |
| GCA CCA GAG GCA CGC AGG AA | ||||
| PDGF-D | XM 002708534.1 | 125 | TGC ACC GGC TCA TCC TCG TCT A | |
| GTC ATC TCG CCG GAG ATT GGC GTT G | ||||
| FGF-2 | XM 002717238.1 | 278 | GGA GAA GAG CGA CCC ACA CAT CA | |
| TAG CCT TCT GCC CAG GTC CTG TT | ||||
| IGF-1 | NM 001082026.1 | 194 | TCT GCG GTG CTG AGC TGG TG | |
| TGC CTT TGC CGG CTT GAG GG | ||||
| Apoptosis | Bcl2 | DQ 529234.1 | 233 | GTG GGA TAC TGG AGA TGA AGA |
| GAC GGT AGC GAC GAG AGA | ||||
| Bax | XM 002723697.1 | 400 | CCA AGA AGC TGA GCG AGT G | |
| TTC CAG ATG GTG AGT GAG G | ||||
| caspase-3 | NM 001082280.1 | 489 | CAA TGG ACT CTG GGA AAT | |
| GCA AGC CTG AAT AAT GA |