Figure 3.
Detection of total fungi, Microdochium and Trichoderma in salmon egg samples by quantitative PCR. Total fungal community was detected using ITS4-ITS9 primers, Microdochium lycopodinum/Microdochium phragmitis species were detected by MPF-MPR primers, and Trichoderma species were detected by ITS1TrF-ITS4TrR primers. The concentration of DNA template of each sample was normalized at 5 ± 1 ng·μL−1. Closed and open circles indicate DNA samples extracted from Saprolegnia-infected (diseased) or healthy samples, respectively. The lane on the left is the size marker and the band size (bp) is indicated next to each band.