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. 2016 Jan 21;17(1):140. doi: 10.3390/ijms17010140

Figure 4.

Figure 4

In vitro activities of Microdochium and Trichoderma isolates. (a) Dual culture of Microdochium isolate 749F1 or Trichoderma isolate 762F1b with Saprolegnia diclina 1152F4 on 1/5th strength potato dextrose agar (1/5PDA). S. diclina and the fungal isolates were pre-grown on 1/5PDA. A hyphal plug of S. diclina was inoculated on the left side of the fresh 1/5PDA and a hyphal plug of Microdochium isolate 749F1 or Trichoderma isolate 762F1b was inoculated on the right side. The dual cultures were incubated for six days at 20–25 °C. The black arrows indicate S. diclina plugs and the white arrows indicate Microdochium or Trichoderma plugs; (b) Microscopic pictures of the hyphal interaction between Trichoderma and S. diclina. The black arrows indicate hyphae of S. diclina. The white arrows indicate the coiling of Trichoderma hyphae around S. diclina hyphae (top pictures) or the formation of papilla-like structure of Trichoderma hyphae around S. diclina hyphae (bottom pictures). Scale bars represent 10 μm.