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. Author manuscript; available in PMC: 2017 Jan 25.
Published in final edited form as: Dev Cell. 2016 Jan 25;36(2):190–200. doi: 10.1016/j.devcel.2015.12.022

Figure 2. Mechanism of ANKS4B targeting to the BB.

Figure 2

(A) Constructs of ANKS4B used for CACO-2BBE stable cell line creation. (B-H) Confocal images of 12 DPC CACO- 2BBE monolayers stably expressing the various EGFP-tagged ANKS4B constructs outlined in A. Boxed regions denote areas in zoomed panels for each figure. Dashed lines represent the location where x-z sections were taken; x-z sections are shown below each en face image. Scale bars, 10 μm. (I) Scatterplot quantification of the BB:cytosol ratios of EGFP signal for all EGFP-tagged ANKS4B constructs. EGFP alone is included as a negative control. Bars indicate mean and SD. *p < 0.0001, t test.