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. Author manuscript; available in PMC: 2017 Jan 25.
Published in final edited form as: Dev Cell. 2016 Jan 25;36(2):190–200. doi: 10.1016/j.devcel.2015.12.022

Figure 6. ANKS4B is required for IMAC assembly and localization at microvillar tips.

Figure 6

(A) Confocal images of 12 DPC CACO-2BBE cells stably expressing either a scramble shRNA construct or an shRNA targeting ANKS4B, stained for F-actin (magents) and either CDHR2, CDHR5, USH1C, or MYO7B (green). Boxed regions denote the area in zoomed image panels. Scale bars, 15 μm. (B) Western blot analysis of endogenous CDHR2, CDHR5, USH1C, MYO7B, and actin levels in lysates from 20 DPC scramble control, or two independent shRNA ANKS4B KD stable CACO-2BBE lines. GAPDH was used as a loading control. (C) Quantification of endogenous CDHR2, CDHR5, USH1C, MYO7B and actin levels in lysates from 20 DPC scramble control, or two independent shRNA ANKS4B KD stable CACO-2BBE lines. Values are expressed as expression level relative to scramble control cell line.