Figure 5.

CK2 inhibition with TBCA induces ERα expression in ER (+) T47D and ER (−) MDA-MB-231 breast cancer cells. (A) T47D and (B) MDA-MB-231 cell were incubated for 24 h in the absence (Con) or presence (TBCA) of 25 μM TBCA. Cells were collected and stained with αERα antibody and fluorescent secondary antibody. Fluorescent staining was assessed by flow cytometry. Background staining was established using secondary antibody alone. Percentage values represent the ratiometric increase in ERα associated fluorescence in the presence of TBCA.