Skip to main content
. Author manuscript; available in PMC: 2017 Jan 25.
Published in final edited form as: Dev Cell. 2016 Jan 25;36(2):139–151. doi: 10.1016/j.devcel.2015.12.019

Figure 3. Wacky is a negative regulator of basal autophagy in Drosophila.

Figure 3

(A) Control (y w FRT19A) and wackyA second instar larval fat body expressing UAS-mCherry-Atg8a (to mark autophagosomes) and UAS-LAMP1-GFP (to mark lysosomes) with the fat body-specific Cg-GAL4 driver. Scale bars are 10 μm.

(B) wackyA clones, marked yellow, in wandering third instar larval fat body expressing UAS-mCherry Atg8a and LAMP1-GFP with Cg-GAL4 driver. Scale bars are 20 μm.

(C) Quantification of autophagosome and lysosome numbers from (A) (n=4), and (B) (n=5). Error bars are SEM and ** is p<0.02.

(D) Cg-GAL4-driven expression of UAS-lacZ as control or UAS-Atg1-RNAi to suppress autophagy induction in third instar larval fat body with wackyA clones marked yellow. Scale bars are 20 μm.

(E) Western blot for Ref(2)P/p62 with fat body protein lysates from third instar larval wackyB ; wacky-gr (control) and wackyB.

See also Figure S3.