Figure 4. Wacky functionally interacts with mTOR in Drosophila.

(A) Western blot for dTOR-dependent phosphorylation of S6k, 4E-BP, and Akt with fat body protein lysates from third instar larval wacky^B ; wacky-gr (control) and wacky^B.

(B) ERG traces from 2-day old whole-eye clones of control (FRT40A) (n=5), dTOR ^P (n=5), and wacky^A (n=4), with quantification for ERG amplitude and Off transient. Error bars are SEM and ** p<0.02.

(C) Images of FRT40A (control), wacky^AFRT19A, and dTOR ^P FRT40A larvae at 4.5 days after egg laying (AEL).

(D) Images of whole eye clones of 2-day old control (FRT40A), wacky^A, and dTOR ^P.

(E) wacky^A and dTOR ^P mutant follicle cell clones, marked yellow, in stage 10 egg chambers. Scale bars are 20 μm.

(F) wacky^A and dTOR ^P mutant follicle cells, marked yellow in stage 14 egg chambers. Scale bars are 20 μm.

See also Figure S4.