FIG 1.

Low-dose intestinal infection with T. muris alters the lung immune microenvironment. (A) Enumerated cecal worm burdens. (B) Lung mRNA expression of Il5, Ifng, and Il10 normalized relative to Actb. (C) ELISA of IL-5, IFN-γ, and IL-10 from ex vivo unstimulated and polyclonally stimulated lung cell cultures. (D) ELISA of IFN-γ from T. muris antigen (Ag)-stimulated lung cell cultures. (E) Lung histology visualized using ×20 magnification and total and differential bronchoalveolar lavage (BAL) fluid counts. Data are means ± SEM, pooled from 3 independent experiments (n = 6 mice per group) (A to E). Sections are representative of 3 independent experiments (E). Ctrl, control; Tm, T. muris infected; nd, not detected; Rel., relative.