FIG 3.

Low-dose T. muris infection induces expression of IL-10 in lung myeloid cells. (A) Total lung IL-10⁺ cell frequencies and absolute numbers in Rag1^−/− Vert-X (IL-10 reporter) mice either uninfected or infected with low-dose T. muris. SSC, side scatter. (B) Frequencies and absolute numbers of live CD45⁺-gated CD11b⁻ CD11c⁺ and CD11b⁺ CD11c⁺ cell populations comprising the total IL-10⁺ cells in the lung. (C to E) Surface marker analysis of Ly6G versus Ly6C (C), Siglec F versus F4/80 (D), and MHC-II versus CD11c (E) of CD11b⁺ CD11c⁺ IL-10⁺ gated cells. (F) Gating of total CD11c⁺ B220⁺ cells and IL-10⁺ frequencies. Data are means ± SEM, representative of 3 independent experiments (n = 5 to 6 mice per experiment) (A to F). Ctrl, control; Tm, T. muris infected.