Time dependence of low-dose T. muris-mediated suppression of house dust mite (HDM) antigen-induced allergic airway inflammation. (A) Time course of low-dose infection either before (Tm d−10) or concurrent (Tm d0) with intranasal (i.n.) sensitization with HDM antigen. (B) Total bronchoalveolar lavage (BAL) fluid cell and BAL fluid eosinophil counts. (C) PAS-stained sections of lungs, visualized using ×20 magnification. (D) Lung mRNA expression of Ifng and Il10 (relative to Actb). Data are means ± SEM, representative of 3 independent experiments (n = 3 to 5 mice per experiment) (B to D). Sections are representative of 3 independent experiments (C). Ctrl, control; Tm, T. muris infected; nd, not detected; Rel., relative.