FIG 4.

Butiril-cAMP restores the ability of BPE005-transfected LX2 cells to secrete MMP-9 and inhibits TGF-β1 secretion. (A and B) LX-2 cells were transfected with BPE005 plasmid DNA (pBPE005) or with pcDNA3-c-myc (Ctrl.) in the presence or absence of B2cAMP. At 24 h posttransfection, MMP-9 production was assessed by zymography (A) and TGF-β1 secretion by ELISA (B). (C) Cells were infected with B. abortus (Ba) at an MOI of 1,000 in the presence or absence of B2cAMP. At 24 h after infection, supernatants were harvested and MMP-9 levels were determined by zymography. Data are given as the means ± SEM of results from experiments performed in duplicate. Data shown are from a representative experiment of three performed. ***, P < 0.001 (versus nontransfected [N.T.]) or transfected and treated [pBPE005, B2cAMP]).