Single-system strains and null growth on LB agar plates. Overnight cultures of wild-type O395, null (EPV102), vFeo (EPV104), vFbp (EPV115), vVct (EPV103), and vVib (EPV126) were streaked on unsupplemented LB agar plates (A) or LB agar plates supplemented with heme (B). Plates were incubated overnight at 37°C.