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Growth of the V. cholerae O395 hemA mutant. (A) Overnight cultures of EPV134 (O395 ΔhemA) and wild-type O395 were diluted 1:200 in LB or LB supplemented with heme. Growth was determined by monitoring A650 over the course of 10 h. (B) Overnight cultures of EPV134 bearing empty vector (pWKS30) or pHemA were diluted 1:200 in LB, and growth was monitored as described above. The data represent the means from biological triplicates.
