TABLE 2.
Strains and plasmids
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| V. cholerae | ||
| O395 | Classical biotype | 69 |
| ARM591 | O395 ΔvibB fbpA::cam | 10 |
| ARM592 | O395 ΔvibB fbpA::cam vctP::gent | This study |
| EPV6 | O395 ΔvibB fbpA::cam vctP::gent feoABC::kan | This study |
| EPV102 (null) | O395 ΔvibB fbpA::cam vctP::gent feoABC::kan ΔviuA | This study |
| EPV104 (vFeo) | O395 ΔvibB fbpA::cam vctP::gent viuA | This study |
| ALV101 | O395 ΔvibB | 10 |
| EPV105 | O395 ΔvibB ΔviuA | This study |
| EPV113 | O395 ΔvibB ΔviuA vctP::gent | This study |
| EPV115 (vFbp) | O395 ΔvibB ΔviuA vctP::gent feoABC::kan | This study |
| CFV1 | O395 feoB::tmp ΔvibB fbpA::cam | 10 |
| EPV103 (vVct) | O395 feoB::tmp ΔvibB fbpA::cam ΔviuA | This study |
| EPV111 | O395 feoABC::kan | This study |
| EPV114 | O395 feoABC::kan vctP::gent | This study |
| EPV126 (vVib) | O395 feoABC::kan vctP::gent fbpA::cam | This study |
| EPV134 | O395 ΔhemA | This study |
| N16961 | El Tor biotype | 70 |
| E. coli | ||
| DH5α | Cloning strain | 71 |
| DH5α λpir | Cloning strain | 71 |
| SM10 λpir | Conjugation strain | 72 |
| Plasmids | ||
| pWKS30 | Cloning vector | 73 |
| pHM5 | Suicide vector pGP704 carrying sacB | 74 |
| pCVD442N | pCVD442 with a NotI adapter inserted in the SacI site | 10 |
| pCGM120 | pCVD442N with V. cholerae sequences for vctP::gent | This study |
| pSfeoΔ::kan | pCVD442N with V. cholerae sequences for feoABC::kan | This study |
| pSviuA | pCVD442N with V. cholerae sequences for deleting viuA | This study |
| pShemA | pCVD442N with V. cholerae sequences for deleting hemA | This study |
| pSfbpA::cam | pCVD442N with V. cholerae sequences for fbpA::cam | 10 |
| pSvibBΔ | pCVD442N with V. cholerae sequences for deleting vibB | 10 |
| pHemA | V. cholerae hemA cloned into pWKS30 | This study |