FIG 3.

Analysis of PhtD expression levels in HT mutant strains grown in CDM-TPEN. mRNA (A) and protein (B) expression levels are shown, normalized to the levels in the ΔadcA ΔphtABE strain. For mRNA, measurements were made relative to 16S rRNA. Strains were compared to the ΔadcA ΔphtABE mutant (striped bars) by one-way ANOVA using Dunnett's posttest, and statistically significant differences, where present, are indicated (****, P < 0.0001). The results (± the SEM) are representative of three independent experiments. WT, wild type. (C) Representative Western blot for the determination of PhtD protein expression.