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. 2016 Jan 25;84(2):395–406. doi: 10.1128/IAI.00870-15

FIG 1.

FIG 1

Expression and cellular localization of Lactobacillus-produced VHH fragments. Detection of TcdB-neutralizing VHH fragments expressed by engineered L. paracasei BL23 strains was performed by immunoblotting of the cell pellet (C) and supernatant (S) fractions. (A) Expression of secreted anti-TcdB VHH fragments by strains KK442 (VHH-B2; 15.20 kDa), KKA440 (VHH-E2; 16.25 kDa), KKA382 (VHH-G3; 16.24 kDa), KKA441 (VHH-D8; 16.74 kDa), and KKA101 (negative control [Neg]). (B) Expression of cell wall-anchored anti-TcdB VHH fragments by strains KK413 (VHH-B2; 39.78 kDa), KKA415 (VHH-E2; 40.78 kDa), KKA416 (VHH-G3; 40.77 kDa), KKA418 (VHH-D8; 41.28 kDa), and KKA101 (negative control). VHH fragments were detected using an anti-E-tag antibody followed by HRP-conjugated anti-mouse immunoglobulins. Arrows indicate the protein bands of expected size. Some degradation of the VHH fragments was seen for the cell wall-anchored constructs due to the crude method for lysing the cells.