TABLE 1.

Characteristics of the mutants^a

Characteristic	RC2-SM	RC2-all2Δ	RC2-all2Δ+PACT1-ALL2	RC2-all1Δall2Δ
Mean capsule diam (μm) ± SEM
In vitro	1.2 ± 0.8	1.0 ± 0.4	1.3 ± 0.65	1.5 ± 0.43
In vivo	10.8 ± 2.6	11.7 ± 2.7	11.8 ± 1.5	ND
Presence of melanin	Yes	Yes	Yes	Yes
Mean growth period (h) ± SEM at:
pH 4	2.3 ± 0.2	2.8 ± 0.1	2.8 ± 0.1	ND
pH 7	2.9 ± 0.8	2.4 ± 0.7	2.5 ± 0.6	ND
Mean phagocytosis index ± SEM	93.2 ± 16.3	113.2 ± 20.3	72 ± 8.9	109.6 ± 32.4
Zone size (mm) on:
YNB + disc containing 5 μl of 30% H2O2	57	65	58	72
MM + disc containing 5 μl of 30% H2O2	54	62	58	64
SAB + disc containing 5 μl of 30% H2O2	30	40	34	38
Glucuronic acid content (mg/cell)	1.40 × 10−13	1.57 × 10−12	5.32 × 10−14	ND

^aSM, smooth; SEM, standard error of the mean; MM, minimal medium; SAB, Sabouraud broth; ND, not determined.