Fig. 3.

Discovery of DUX4-interacting motifs by SELEX. a PCR assay after indicated rounds of DNA pulldown. Filled triangle indicates the specific band; open triangle indicates primer dimers. Note that the amplified product is enriched over primer dimers by the last cycle shown. b Selection bias for each base at each position (base frequency in the pulled down sequence/base frequency in the control). c Luciferase reporter assays for the top four SELEX-identified sequences. d Direct band shift assays for the top 4 SELEX motifs. e Competitive activity of the top four SELEX motifs. Each unlabeled SELEX probe was used to compete the shift of a FAM-labeled probe bearing the CT motif