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. 2016 Jan 28;5:2. doi: 10.1186/s40164-016-0032-7

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© Liew et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Creation of the Tet-inducible JAK2V617F-expressing cell line D9. a A schematic representation of the Tet-inducible construct containing the JAK2V617F gene tagged with a V5 epitope. b Confirmation of JAK2V617F induction. Equal amounts of protein prepared from clones treated with Tet or vehicle alone for 24 h were subjected to immunoblotting analysis using an anti V5 antibody to detect JAK2V617F