Fig. 4.

Identification of AIM2 as a downstream target of JAK2V617F. a Presumptive JAK2V617F induction was verified by qRT-PCR analysis for JAK2 at the indicated time points after Tet induction, as shown in the lower panel. V5-tagged JAK2V617F induction was observed by immunoblotting analysis (upper panel). Note that qRT-PCR did not discriminate between endogenous JAK2 and Tet-induced V5-tagged JAK2V617F mRNA, thus the rate of JA2V617F mRNA induction may be underrepresented. b A heat map showing the expression of genes in the cytosolic DNA sensing pathway in D9 and UT-7/GM/TetR (control) cells at the indicated time points. RNA samples from three independent experiments were analyzed. Green indicates lower gene expression and red indicates higher gene expression. c qRT-PCR analysis of AIM2 expression using the cDNA analyzed in (b). d A model explaining the potential roles of how AIM2 and IL1B might act downstream of JAK2V617F to contribute to myelofibrosis