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. 2016 Jan 28;16:45. doi: 10.1186/s12885-016-2076-9

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© Fang et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Lentivirus-mediated stable Talin-1 (TLN-1) knockdown in the minimally metastatic HCC MHCC-97 L cells. Blank: non-transduced MHCC-97 L cells; TLN-1 shRNA: MHCC-97 L cells transduced with lentivirus-mediated TLN-1 shRNA; Scramble shRNA: MHCC-97 L cells transduced with lentivirus-mediated scramble shRNA. a According to fluorescence microscopy, transduction efficiency in MHCC-97 L cells (the percentage of GFP-positive cells) was >70 % at 72 h (magnification: ×200). b TLN-1 mRNA expression levels were determined by real-time RT-PCR at 72 h after transduction, with GAPDH used as an internal control. c TLN-1 protein expression levels were determined by Western blot at 72 h after transduction. β-actin was used as an internal control. Data are mean ± Standard deviation (SD). **P < 0.01 vs. scramble shRNA