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. 2016 Jan 28;16:33. doi: 10.1186/s12870-016-0715-6

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© Li et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — BR biosynthesis capacity in mutant defective in Dwarf (d ^im), wild type (WT) and Dwarf overexpressing lines (DWF:OX2 and DWF:OX3). a Confirmation for the presence of transgene in the selected lines by PCR. M, 100 bp marker; V+, transformed vector; V-, genomic DNA of WT plants; 1–7, selected plant lines. b–d Dwarf transcript and accumulation of BR biosynthetic precursors. Leaves were harvested at 45d after germination for the gene expression and BRs content analysis. Data are the means ± SD of four independent biological samples. Means denoted by the same letter did not differ significantly according to Tukey’s test (P < 0.05). CS, castasterone; 28-norCS, 28-norcastasterone; BL, brassinolide