Fig. 6.

Complementation of yeast alg3-1 mutant strain (YG170) with the Arabidopsis ALG3 variants. The Arabidopsis ALG3 (AtALG3) open-reading frames (ORFs) were amplified from cDNAs of wild-type plants or the cce2/cce3 mutants, cloned into yeast expression vectors, and transformed into yeast strain YG170. a Scheme of expression vector constructs of the indicated ORFs under the control of a galactose-inducible promoter (GAL1_pro) and a cytochrome c terminator (CYC1_TT). b RT-PCR validation of gene expression from the transfected constructs. Actin 1 (ACT1) was used as an amplification/loading control. c Complementation of the alg3-1 mutant yeast strain YG170 was determined by comparing growth without or with galactose-induced expression of the indicated constructs under non-permissive temperature (30 °C). Growth at permissive temperature (25 °C) was used to show the viability of the yeast cells. Five serial dilutions were performed in all cases. d Complementation was further tested by reducing expression of the AtALG3 or the cce3 variant (by decreasing galactose concentrations from 2 % to 0.02/0.002 %)