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Impact of freezing delay time on level of (A) ascorbate, (B) choline, (C) creatine, and (D) glutathione. Metabolite integrals from samples subject to 15, 30, 60, 90, and 120 min freezing delay time compared with samples frozen immediately (0 min). * and ** indicates that the level is significantly different from the sample frozen after 0 min (*p < 0.05, **p < 0.01).
