Table 3.

Metabolic effect of prolonged experiment time.

Metabolite	ppm	1.5 h	Adj. p-value
Glucose	4.65	21 ± 20%	0.006**
Ascorbate	4.53	−4 ± 6%	0.078
Lactate	4.15	0 ± 7%	0.903
Tyrosine	3.98	3 ± 6%	0.08
Glycine	3.56	8 ± 7%	0.006**
Myoinositol	3.54	7 ± 11%	0.08
Taurine	3.42	0 ± 5%	0.903
Glycerophosphocholine	3.23	−15 ± 12%	0.001**
Phosphocholine	3.22	−4 ± 6%	0.08
Choline	3.21	11 ± 13%	0.011*
Creatine	3.03	0 ± 6%	0.903

Percentages (average ± SD) were calculated relative to the metabolite levels (integrals) from the initial experiment. Adjusted p-values in bold indicates that the level is significantly different from the sample frozen after 0 min (*adjusted p < 0.05, **adjusted p < 0.01).