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. 2015 Dec;10(12):1989–1996. doi: 10.4103/1673-5374.172317

Figure 5.

Figure 5

Salidroside increased the protein level of Nrf2 and HO-1 and up-regulated HO-1 mRNA expression in the ischemic cortex.

(A) Representative images of western blot assay for Nrf2, HO-1. (B) Quantification of optical density for Nrf2, normalized to β-actin. (C) Quantification of optical density for HO-1, normalized to β-actin. (D) Effect of salidroside on relative HO-1 mRNA level in the ischemic brain. Salidroside (30 mg/kg) significantly reduced HO-1 mRNA expression compared with the vehicle group. Data are expressed as the mean ± SD (n = 3 for western blot assay; n = 5 for reverse transcription-PCR test; one-way analysis of variance, followed by the least significant difference post hoc test). **P < 0.01, vs. sham; #P < 0.05, ##P < 0.01, vs. vehicle. Sham: Sham opera-tion group; vehicle: 0.9% saline-treated group; Sal: 30-mg/kg salidroside group; Nrf2: nuclear factor erythroid 2-related factor 2; HO-1: heme oxygenase-1. Immunoblots were repeated at least twice and no less than three samples were used per test.