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. 2016 Jan 26;4:e1640. doi: 10.7717/peerj.1640

Figure 7. Protein levels of Akt, p-Akt(Ser473) and active caspase-3.

Figure 7

NPCs were divided into four groups. Group A was treated with 75 ng/ml IL-1β. Group B was treated with 75 ng/ml IL-1β with the pretreatment of 1 μM E2 and 200 μM RSV for 30 min. Group C was treated with 75 ng/ml IL-1β with the pretreatment of 1 μM E2, 200 μM RSV, and 1 μM ICI for 30 min. Group D was treated with 75 ng/ml IL-1β with the pretreatment of 1 μM E2, 200 μM RSV and 50 μM LY294002(PI3K/Akt inhibitor). All groups were incubated for 24 h in the serum-free medium without phenol red. * p < 0.05, by one-way analysis of variance (ANOVA) accompanied by pairwise comparison using SNK-q test. NPCs, nucleus pulposus cells; IL-1β, interleukin-1β; E2, 17β-estradiol; ICI, ICI182780; RSV, resveratrol; LY, LY294002, 50 μM; mean ± SD (standard deviation); n = 6.