Table 2. Metabolites detected by GCMS in lysates of HMCL-7304 myotubes.
| Metabolite | Retention time (min) | Derivatives formed | HMDB number |
|---|---|---|---|
| L-Lactic acid | 13.33 | 2TMS | 00190 |
| Caproic acid | 13.66 | 1TMS | 00535 |
| Glycolic acid | 13.83 | 2TMS | 00115 |
| Hydroxylamine | 15.22 | 3TMS | 03338 |
| L-Leucine | 20.68 | 2TMS | 00687 |
| L-Isoleucine | 21.42 | 2TMS | 00172 |
| L-Valine | 18.74 | 2TMS | 00883 |
| Ethanolamine | 20.48 | 3TMS | 00149 |
| Phosphate | 20.81 | 3TMS | 01429 |
| L-Isoleucine | 21.42 | 2TMS | 00172 |
| Glycine | 21.84 | 3TMS | 00123 |
| Glyceric acid | 22.76 | 3TMS | 00139 |
| L-Threonine | 24.64 | 3TMS | 00167 |
| L-5-oxoproline | 28.76 | 2TMS | 00267 |
| Glycerol 3-phosphate | 35.92 | 4TMS | 00126 |
| Citric acid | 37.37 | 4TMS | 00094 |
| Myristic acid (14:0) | 37.57 | 1TMS | 00806 |
| D-Glucose | 39.21 39.65 40.11 |
5TMS 5TMS; 1MOX 5TMS; 1MOX |
00122 |
| Palmitic acid (16:0) | 42.36 | 1TMS | 00220 |
| Myo-Inositol | 44.21 | 6TMS | 00211 |
| Oleic acid ((9Z)-18:1) | 46.24 | 1TMS | 03229 |
| Vaccenic acid ((11E)-18:1) | 46.39 | 1TMS | 03231 |
| Stearic acid (18:0) | 46.78 | 1TMS | 00827 |
| (1-Myristoylglycerol) | 49.93 | 2TMS | 11561 |
| (2-Palmitoylglycerol) | 52.15 | 2TMS | – |
| (1-Palmitoylglycerol) | 52.56 | 2TMS | 31074 |
| (2-Stearoylglycerol) | 54.34 | 2TMS | – |
| (1-Stearoylglycerol) | 54.75 | 2TMS | 31075 |
| Cholesterol | 60.72 | 1TMS | 00067 |
Notes:
TMS, trimethylsilyl group; MOX, O-methoxyimine group.
HMDB, Human Metabolome Database (http://www.hmdb.ca/).
Metabolites in (parentheses) are contributed to by leaching from certain laboratory plasticware and were not included in the multivariate data analysis.