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Immunoprecipitation (TGFβRII) and immunoblotting (3-NT) of protein tyrosine nitration on TGFβRII. A, Negative control: antibodies only; Sham control: tissue without I/R; I/R: tissue with I/R. There is a clear nitrated band at about 70 kDa: the nitrated TGFβRII. B, relative intensity of the nitrated band. *p<0.05 vs. Sham control. N=4/ group.
