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. Author manuscript; available in PMC: 2016 Jan 28.
Published in final edited form as: Calcif Tissue Int. 2015 Feb 25;96(5):465–475. doi: 10.1007/s00223-015-9967-7

Fig. 1.

Fig. 1

The expression of LNX1 and LNX2 is up-regulated during osteoclast differentiation. Bone marrow macrophages (BMMs) were cultured with M-CSF alone or M-CSF plus RANKL for 2 and 4 days to generate macrophages (BMM), mononuclear pre-osteoclasts (p-OC) and mature osteoclasts (mOC), respectively. At these time points, more than 80% of cells were mononuclear pre-OCs and multinucleated mOCs in the respective cultures. Total RNAs were purified from three independent cultures of BMM, pOC, and mOC, respectively. Quantitative real-time RT-PCR analysis of the expression of LNX mRNA during osteoclast differentiation was performed using TaqMan assay primers from Life Technologies. The representative data from three experiments were presented as mean ± S.D. (A) data were analyzed using the ΔCt method. ** p < 0.01, *** p < 0.001 vs BMM by one-way ANOVA. (B) The relative levels of LNX1 and LNX2 cDNAs in BMM and mOC were analyzed using the delta ΔCt method. *** p < 0.001 vs LNX1 by a 2-tailed Student’s t test.