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. 2016 Jan 26;12(1):849. doi: 10.15252/msb.20156590

Figure 5. Relay devices can initiate and propagate signals through population‐level positive feedback.

Figure 5

  • A–E
    All cells express chromosomal constitutive mRFP1 and are plated on membranes printed with hydrophobic grids. Cells transformed with constitutive control (pPRYFPPRCFP) and plated along the edge of the membrane provide a standard for normalization and minimize edge effects. (A–C) Images at t = 1,500 min of relay devices co‐transformed with double receiver (pR33S175) and arranged in alternating stripes with a width of two grid squares. Blue arrows indicate stripes of pLux76LasI while purple arrows indicate pLas81LuxI. Stripes with no arrow contain double receiver with empty vector. About 15 μl of 20 μM 3OC12HSL was plated in a stripe above the first stripe of cells at t = 0. (A) Alternating stripes of pLux76LasI and pLas81LuxI. (B) Alternating stripes of pLas81LuxI and empty vector. (C) Alternating stripes of pLux76LasI and empty vector. (D) Images at the time points indicated of cells co‐transformed with double receiver (pR33S175) and either pLux76LasI or pLas81LuxI and plated in a checkerboard pattern with the addition of a mixed population of both cell types in an 8 × 8 square in the center. (E) Images at the timepoints indicated of a layout identical to (D) except for the absence of a mixed population.

Source data are available online for this figure.