Figure 1.

PCL localizes to the primary cilia of neuronal cells in the brain. (A) Confocal imaging indicates PCL expression in the neuronal cells in the cerebral cortex, hippocampus and thalamus. (B) PCL localizes to the primary cilia of hippocampal neurons in a primary culture. (C) PCL localizes to the AC8⁺ neuronal primary cilia in the mouse cortex and hippocampus. Insets show the cilia indicated by arrows. PCL was stained by antibody 83430. Neurons were detected by neuronal marker NeuN. AC3 was used to mark neuronal primary cilia. AC8 is a new neuronal cilium marker identified in this study.