Figure 4.
cAMP is reduced in Pkdl−/− mouse brain tissues. (A) The cAMP levels in adult Pkdl−/− mouse brains were reduced by ∼27% compared with the WT littermates. (B) Consistently, western blot revealed that pCREBS133 was also significantly reduced in the same brain tissues. (C) The pCREBS133 protein levels were normalized to total CREB protein in each brain tissue. The WT brain tissues were set at 100%, as reference. Error bars represent standard deviation (n = 5). The significance was calculated by Student's t-test (P < 0.01). Three independent experiments were performed. (D) There were no obvious changes in phosphorylation of CaMKII, Akt or MAPK in Pkdl−/− brain tissues compared with WT mice.