Skip to main content
. Author manuscript; available in PMC: 2016 Feb 3.
Published in final edited form as: Biochemistry. 2015 Jan 17;54(4):1043–1052. doi: 10.1021/bi501350j

Figure 2. Enzyme assembly and covalent FAD incorporation.

Figure 2

Proteins were separated on a 12 % SDS-PAGE gel and visualized by either Coomassie Blue staining (top) or UV excitation (bottom). Samples from left to right: low molecular weight standards, followed by enriched membrane preparations containing no Sdh (E. coli DW35), wild-type Sdh (WT), SdhA-H45A, -R286A, -R286K, -R286Y, -H242A and -H242Y enzymes.