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. 2016 Jan 28;11(1):e0147682. doi: 10.1371/journal.pone.0147682

Fig 2. Blocking the PI3K pathway does not influence cell growth or signaling.

Fig 2

A) CHP-212 and SK-N-AS cells were treated with 500nM of the MEK inhibitor MEK162 or the PI3K inhibitor BKM120 for 1 hour and next lysed and subjected to Western blot. Phosphorylation levels of AKT, ERK and S6 were detected by specific anti-phospho antibodies. Loading was verified by specific antibodies to total AKT, ERK and anti—tubulin. B) CHP-212 and SK-N-AS cells were treated with 500nM of MEK162 or BKM120 or combinations thereof as indicated for 96h. Then, cell growth was assessed by Cell titer Glo. C) Same as B) but the AKT1/2/3 inhibitor GSK690693 was used instead of BKM120. D) Same as C) but the AKT1/2/3 inhibitor MK2206 was used instead of GSK690693.