Table 1. qPCR primers and primer efficiency.
Forward Primer (5’-3’) | Reverse Primer (5’-3’) | Primer Efficiency | |
---|---|---|---|
B-actin (Reference) | CATCCAAGCTGTACTCTCCTTG | GGAAGAGCGTAACCTTCGTAG | 1.92 |
Comp115309 (Cytochrome P450) | CGAGAAATGCGACCTATTCTCAG | ACACAGTCTTGGTCTTTCTTGAG | 1.98 |
Comp105889 (Cuticular protein) | CTCCAGTGGTTCCGTTATTACAC | AGCGTAGTCGTGGAAATGTTG | 1.94 |
Comp114026 (Glutathione synthetase) | CAGAGCAGGGTATGAACCTAATC | CCAGCCAAGTGATACTGAATCG | 1.97 |