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. 2015 Jun 5;27(2):417–427. doi: 10.1681/ASN.2014100998

Figure 5.

Figure 5.

Transcriptional activation of Ret promoter by Wnt/β-catenin signaling. (A) Schematic structure of the mouse Ret promoter with four putative Tcf/lef1-binding sites or Wnt-responsive elements (R1–R4). (B) Chromatin immunoprecipitation demonstrates the specific amplification of the R4 site immunoprecipitated with β-catenin antibodies, not the control IgG, from either E14.5 WT embryonic kidneys or L cells. (C) Luciferase assay results show specific response of WT Prw-luc, but not the mutant Prm-luc constructs, co-transfected with the persistent active β-catenin and Lef1 constructs. (D) Dose-dependent activation of Prw-luc construct treated with Wnt3a. (E) Dose-dependent activation of Prw-luc construct treated with Wnt agonist lithium chloride. *P<0.05; **P<0.01.