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. 2015 Jun 8;27(2):482–494. doi: 10.1681/ASN.2014111107

Figure 2.

Figure 2.

Acute albumin overload results in decreased PT uptake. (A) An intravital microscopy single time point image showing TR-RSA fluorescence in the S1 region of a control and protein overload animal, 20–30 min after TR-RSA infusion. Note the reduced uptake in the protein overload animal. Bar, 20 μm. (B) Box plot showing the quantification of TR-RSA uptake in all surface PTs of control (n=3 rats, 157 fields quantified) and protein overloaded (n=8 rats, 176 fields quantified) rats. This analysis showed a significant reduction in albumin uptake, P<0.01 (KaleidaGraph, Student’s t-test). (C) Quantification of the TR-RSA uptake in only S1 PTC identified reduced uptake in the protein overload animals. Each data point represents an individual measurement from control (n=36 from three rats) or protein overload (n=83 from seven rats) S1 PT. The differences in the slopes of these lines was found to be significant, P<0.001, GraphPad Prism.