Table 1.

Kidney cortex proteins altered by protein overload

Gene ID Protein ID	P Value	Fold Change	Description (UniProt)
Albu Serum Albumin	<0.001	+1.8	The main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
Calr Calreticulin	<0.01	−1.3	Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER.
Dab2 Disabled homolog 2	>0.02	+1.2	Adapter protein that functions as clathrin-associated sorting protein (CLASP) required for clathrin-mediated endocytosis of selected cargo proteins. Can bind and assemble clathrin, and binds simultaneously to phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2) and cargos containing non-phosphorylated NPXY internalization motifs, such as the LDL receptor, to recruit them to clathrin-coated pits.
Dlat Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex	>0.02	+1.2	The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2, and thereby links the glycolytic pathway to the tricarboxylic cycle.
Tpm3 Tropomyosin alpha-3 chain	0.004	+1.2	Binds to actin filaments in muscle and non-muscle cells. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments.
Prdx2 Peroxiredoxin-2	0.05	+1.2	Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2.
Gm2a Ganglioside GM2 activator	0.05	+1.2	The large binding pocket can accommodate several single chain phospholipids and fatty acids; GM2A also exhibits some calcium-independent phospholipase activity.
Ssbp1 Single-stranded DNA-binding protein	>0.02	+1.2	This protein binds preferentially and cooperatively to ss-DNA. Probably involved in mitochondrial DNA replication.
Asrgl1 Isoaspartyl peptidase/L-asparaginase	>0.02	+1.2	Has both L-asparaginase and beta-aspartyl peptidase activity. Is highly active with L-Asp beta-methyl ester.
Acot13 Acyl-coenzyme A thioesterase 13	>0.02	+1.2	Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH.
Fkbp2 Peptidyl-prolyl cis-trans isomerase	>0.02	+1.2	PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Lyz1 Lysozyme C-1	>0.02	+1.2	Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents. In the intestine they may also have a digestive function.
Btf3 Basic transcription factor 3	0.01	+1.2	When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription.
Cfl2 Cofilin 2	0.01	+1.2	Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.
Dld Dihydrolipoyl dehydrogenase	>0.01	−1.2	Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes. Involved in the hyperactivation of spermatozoa during capacitation and in the spermatozoal acrosome reaction.
Aldh8a1 Aldehyde dehydrogenase family 8 member A1	>0.01	−1.2	Converts 9-cis-retinal to 9-cis-retinoic acid. Has lower activity toward 13-cis-retinal. Has much lower activity toward all-trans-retinal. Has highest activity with benzaldehyde and decanal (in vitro). Highly expressed in adult kidney and liver.
Oxct1 Succinyl-CoA:3-ketoacid coenzyme A transferase 1	>0.01	−1.2	Key enzyme for ketone body catabolism. Transfers the CoA moiety from succinate to acetoacetate. Formation of the enzyme-CoA intermediate proceeds via an unstable anhydride species formed between the carboxylate groups of the enzyme and substrate.
Glud1 Glutamate dehydrogenase 1	>0.01	−1.2	Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle.
Calb1 Calbindin	<0.01	−1.2	Buffers cytosolic calcium. May stimulate a membrane Ca2+-ATPase and a 3′,5′-cyclic nucleotide phosphodiesterase.
Psma5 Proteasome subunit alpha type-5	<0.01	−1.2	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Atp5d ATP synthase subunit delta	0.02	−1.2	Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.
Myl6 Myosin light polypeptide 6	0.02	−1.2	Regulatory light chain of myosin. Does not bind calcium.
Atp6v1b2 V-type proton ATPase subunit B, brain isoform	0.04	−1.1	Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Hspd1 60 kDa heat shock protein	0.04	−1.1	Implicated in mitochondrial protein import and macromolecular assembly. May facilitate the correct folding of imported proteins. May also prevent misfolding and promote the refolding and proper assembly of unfolded polypeptides generated under stress conditions in the mitochondrial matrix.
Pdia3 Protein disulfide-isomerase A3	0.04	−1.1	Catalyzes the rearrangement of –S–S– bonds in proteins.
Pepd Xaa-Pro dipeptidase	0.04	−1.1	Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because of the high level of iminoacids in collagen.
Chdh Choline dehydrogenase	0.04	−1.1	Choline + acceptor = betaine aldehyde + reduced acceptor.
Cat Catalase	0.04	−1.1	Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells.
Pyroxd2 Pyridine nucleotide-disulfide oxidoreductase domain-containing protein 2	0.04	−1.1	Probable oxidoreductase.
Atp5b ATP synthase subunit beta	<0.03	−1.1	Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.
Pdia6 Protein disulfide-isomerase A6	<0.03	−1.1	May function as a chaperone that inhibits aggregation of misfolded proteins.
Chchd6 Coiled-coil-helix-coiled-coil-helix domain-containing protein 6	<0.01	−1.1	Required for maintaining mitochondrial crista morphology, ATP production and oxygen consumption.
Tst Thiosulfate sulfurtransferase	<0.01	−1.1	Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA.
Hadh Hydroxyacyl-coenzyme A dehydrogenase	<0.01	−1.1	Plays an essential role in the mitochondrial beta-oxidation of short chain fatty acids. Exerts its highest activity toward 3-hydroxybutyryl-CoA.
Vdac1 Voltage-dependent anion-selective channel protein 1	<0.01	−1.1	Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis.