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. 2015 Jun 24;27(2):495–508. doi: 10.1681/ASN.2014111108

Figure 3.

Figure 3.

RA signaling is activated in the kidney after IR-AKI in mice. Unilateral IR-AKI was performed in male RARE-hsp68-lacZ reporter mice. (A–F) RARE-dependent β-Gal activity in (A) E18.5 embryonic kidney, (B) uninjured kidneys, and injured kidneys at (C) 12, (D) 24, (E) 72 hours, and (F) day 7 after injury, as indicated. Yellow dotted lines demarcate limits of the OM. (G) Quantification of RARE-hsp68-lacZ reporter activity time course after injury. Percent β-Gal–positive area in the OM: uninjured mice (n=12), 12 hours (n=9), 24 hours (n=13), 72 hours (n=8) and day 7 (n=11) after injury. Kruskal–Wallis one-way ANOVA (P<0.001) using Dunn’s test for multiple comparisons with uninjured controls: ***P<0.001, #P<0.001. (H–P) Cellular localization of β-Gal activity in RARE-hsp68-lacZ reporter kidneys after IR-AKI. β-Gal is pseudocolored in white, other markers as indicated. Timing after injury, as indicated. (H and J) Proximal tubular cell marker, lotus tetraglonolobus lectin (green) and Collagen IV (red). (K–M) Proximal tubular cell injury marker, Kim1 (red). (N and P) Macrophage/dendritic cell marker F4/80 (red). Black scale bars, 500 µm; white bars, 50 µm.