Figure 4.

Representative images of glomerular C3 in Cfh^−/− mice injected with a single dose of CR2-FH. The reduction in glomerular C3 and C3b/iC3b/C3c intensity at 48 hours following a single injection of CR2-FH in Cfh^−/− mice persisted to at least 96 hours. In contrast, no change in glomerular C3d reactivity was demonstrable (see also Supplemental Figure 5). CR2-FH reactivity, detected using the Alexa 594-conjugated rabbit anti-mouse FH polyclonal antibody, was demonstrable at all time points after injection and no reactivity was seen in nontreated in Cfh^−/− mice. Original magnification, ×40.