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. 2015 Jun 8;27(2):509–519. doi: 10.1681/ASN.2014121208

Figure 1.

Figure 1.

ROCK1 is activated in muscles of mice with CKD. (A) A structural diagram of ROCK1. When RhoA is bound to the Rho domain (green), it activates ROCK1. Alternatively, caspase-3 can remove the inhibitory Cys domain (blue) to activate ROCK1 constitutively. (B) Immunofluorescent staining of ROCK1 in myofibers of a normal mouse. Original magnification ×400. (C) ROCK1 protein levels were assessed by western blots in skeletal muscles of control (CTL) and CKD mice. Note the 130-kD fragment of cleaved ROCK1 is present in muscle of CKD mice. (D) ROCK1 activity assay revealed that the phosphorylation of MYPT (p-MYPT) increased in muscles of CKD mice (*P<0.01; n=5). (E) RhoA activity assay demonstrated that CKD does not activate RhoA in muscle.